Journal: Springer Protocols Handbooks

Article Title: Animal Models of Acute Neurological Injuries II

doi: 10.1007/978-1-61779-782-8

Figure Lengend Snippet: Fig. 3. APP staining to show the temporal and spatial analysis of axonal damage after focal cerebral ischemia. Accumulation of amyloid precursor protein (APP) was monitored with a rabbit polyclonal anti-APP antibody ( red ) and compared with staining for normal myelin using a mouse monoclonal anti-MBP ( green ). ( a – c ) Representative confocal images of sections double labeled for MBP and APP in the core of the infarct ( left ). The center is out of view to the left. In the merged images from the edge of the infarct ( right ), arrowheads surround a myelinated bundle containing puncta of APP staining. Scale bars = 100 µ m (adapted with permission and modifi cation from ref. 1 ).

Article Snippet: 1° Antibodies—same as for Western blot protocol: (a) 4-HNE: Monoclonal antibody (JaICA, Cat# MHN-020P). (b) Acrolein: Rabbit polyclonal anti-acrolein (Novus Biologicals Inc., Cat# NB200-556). (c) 3-NT: Nitrotyrosine Mouse IgG2b IgG monoclonal Antibody (GenWay Biotech, Inc. Cat#: 20-321- 175259). (d) Protein carbonyl: Rabbit α -DNP antibody (Chemicon international, Cat: 90451, Chemicon International Oxyblot ® Protein Oxidation Detection Kit S7150 or Sigma Rabbit α -DNP antibody: D9656).

Techniques: Staining, Labeling